After seven studio albums and over 13 years together, Jack and Meg White announced the split on their website last night.
The pair posted an official statement which read: "[The split] is for a myriad of reasons, but mostly to preserve what is beautiful and special about the band and have it stay that way."
The former husband and wife duo stressed that the split was 'not due to artistic differences or lack of wanting to continue'. They also denied health concerns were a factor.
Jon Tolley, owner of Banquet Records in Kingston, called the pair "one of the most iconic bands of the last decade", adding: "Pushing boundaries, sometimes musically, sometimes otherwise, their presence will be missed by many, but I for one am happy they left whilst still on a high.
"Jack will no doubt work on his numerous other projects and their Third Man record label is something to keep an eye on too. Maybe Meg can now get some drum lessons."
There had been concerns surrounding Meg White's health after the band had to pull out of performances in 2007 citing 'acute anxiety' on Meg's behalf.
The White Stripes will continue to put out previously unreleased live and studio recording through Jack White's own record label, Third Man Records.
The band reached the peak of their success with 2003's Elephant which reached top spot in the UK Album Chart and spawned the infectious single, Seven Nation Army, which reached number 7 in the singles chart.
The band released its final album, Icky Thump, nearly four years ago before continuing to branch off in their own directions.
In a final nod to their fans, the band wrote: "“The White Stripes do not belong to Meg and Jack anymore. The White Stripes belong to you now and you can do with it whatever you want.
"Thank you for sharing this experience. Your involvement will never be lost on us and we are truly grateful."